The Operetta CLSTM (Perkin Elmer) high-content analysis system is equipped with one camera and eight LED light sources and several filters (see table1). The instrument features a spinning disk confocal mode and can work with 5x air objectives but also with a 20x or 40x water objective supported with a water immersion kit, which is especially suited for imaging of zebrafish larvae. Currently Harmony version 4.8 is used to operate the high-content imager and to perform automated image analysis. We use the Preci-Scan function to detect zebrafish larvae in the well of interest in order to minimize measurement time and data generation. The software can be trained to detect certain zebrafish phenotypes, which is essential to carry out automated compound screens.

objectives LEDs emission filters
1.25x 355-385 nm 430-500 nm
5x 390-420 nm 470-515 nm
10x 435-460 nm 500-550 nm
20x w 460-490 nm 525-580 nm
40x w 490-515 nm 570-620 nm
530-560 nm 570-650 nm
615-645 nm 655-760 nm
650-675 nm 685-760 nm

Table 1: ZANDR Operetta CLS specs